Hull took the lead midway through the first half through Nicky Forster, but Colchester levelled the scores in the second half through a neat Jamie Cureton strike.
Hull pushed forward midway through the half, but they couldn't find the finish until the 24th minute, as Ian Ashbee collected the ball on the left-hand side of the box and swung in a right-footed cross which found Nicky Forster with space in the area and the Hull frontman placed the ball into the back of the net from close range.
Hull were nearly further in front minutes later as Stuart Elliott swung in a neat cross from the left and Dean Windass fired in a right-footed volley from the right-hand side of the box which flashed past Dean Gerken's right-hand post.
Colchester continued to push forward and balls across the box from Cureton and Karl Duguid in the latter stages of the half should have brought rewards for the visitors, but they failed to get on to the end of them.
Cureton had a good chance early in the second half as Duguid played the ball into him from the right, but the former Reading man couldn't make the most of the space as he blazed the ball over from ten yards.
Colchester levelled the scores on 62 minutes as a long ball from the back landed at the feet of Cureton, who turned and fired in a right-footed shot from just inside the box which flew past Boaz Myhill and into the corner of the net.
Substitute Kevin McLeod nearly had Colchester in front towards the end of the game as he struck a fierce left-footed shot from 30 yards, but Myhill did well to turn the ball around the post.